1. Introduction to Biotechnology,2. Scope of Animal Biotechnology,3. Importance of Animal Biotechnology,4. History of old Animal Biotechnology,5. History of New Animal Biotechnology,6. Animal Biotechnology in India,7. Animal Biotechnology Research Promotion in India,8. Priorities given to Animal Biotechnology in India,9. Cloning Vectors,10. Plasmids,11. Properties of Plasmids,12. Plasmid Types,13. Properties of an Ideal Gene Cloning Vector,14. pBR 322,15. pUC8,16. Ti Plasmid,17. Disarmed Ti Plasmids,18. Bacteriophage Vectors ,19. Lambda Phage Vector,20. Insertional Vectors,21. Replacement Vectors,22. Life Cycle of ƒÜ Phage,23. M13 Bacteriophage Vector,24. Cosmids,25. Phagemids,26. Plant Viral Vector – CaMV,27. Animal Viral Vector – SV40,28. Yeast Artificial Chromosomes (YAC),29. Transposons as Vectors ,30. Restriction Enzymes,31. Enzymes Used in Genetic Engineering,32. RestrictionModification Systems,34. Preparation of Desired DNA,33. Gene Cloning,35. Isolation of Plasmid Vector,36. Insertion of Desired Gene into Vector DNA,37. Introduction of Recombinant DNA into Host Cells,38. Introduction of rDNA into Prokaryotic Cells,39. Transfection-Introduction of rDNA into Eukaryotic Animal Cells,40. Transduction – Introduction of rDNA into Eukaryotic Animal Cells,41. Microinjection – Introduction of rDNA into Eukaryotic Animal Cells,42. Biolistics – Introduction of rDNA into Eukaryotic Animal Cells,43. Electroporation -Introduction of rDNA into Eukaryotic Animal Cells,44. Lipofection – Introduction of rDNA into Eukaryotic Animal Cells,45. Screening and Selection of Recombinants,46. Insertional Inactivation,47. Blue-white Selection,48. Direct Selection,49. Nucleic Acid Hybridization,50. Colony Hybridization,51. Blotting Techniques,52. Southern Blotting,53. Northern Blotting,55. Genomic Library,54.Western Blotting ,56. DNA Probes,57. Polymerase Chain Reaction (PCR),58. Animal Cell and Tissue Culture,59. History of Animal Tissue Culture,60. Requirements for Animal Cell Culture Laboratory,61. Substrates for Animal Cell Culture,62. Liquid Media for Animal Cell Culture,63. Gases for Animal Tissue Culture,64. Maintenance of Aseptic Condition,65. Cell Culture Technique,66. Isolation of Explant,67. Culture of Explants,68. Disaggregation of Explants,69. Cryopreservation – Storage of Animal Cells,70. Large – scale Culture,71. Monolayer Culture,72. Suspension – Culture for Animal Cells,73. Bioreactors,74. Continuous Stirred Tank Bioreactors (CSTB),75 . Air-Lift Bioreactors,76. Organ Culture,78. Artificial Cartilage,77. Artificial Skin,79. StemCells,80. Stem Cell Culture,81. Hybridoma Technology,82. Monoclonal Antibodies (MAbs),83. Transgenic Animal Technology,84. Transgenic Dolly,85. Applications of Transgenic Animals,86. Gene Therapy,87. Bone Marrow Transplantation,88. Liver Transplantation,89. Enzyme Technology,90. The Production of Beta Galactosidase Enzyme,91. Enzyme Immobilization,92. Bioethics,93. Intellectual Property Rights (IPR),94. Patenting of Biotechnological Products,95. Biosafety Guidelines in India,97. Bioremediation,96. Sewage Treatment,98. Role of Genetically Engineered Organisms in Bioremediation,99. Superbug ,100. Phytoremediation,101. Biofuels,102. Ethanol as Biofuel,103. Biogas as Biofuel,104. Aquaculture Biotechnology,105. DOT – ELISA (Enzyme Linked Immuno Sorbent Assay),106. Human Genome Project,107. DNA Finger Printing Techniques,108.Microarray,109. Biochips,110. Bioweapons,Index,University Questions,
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