Description

1. Introduction to Biotechnology
2. Scope of Animal Biotechnology
3. Importance of Animal Biotechnology
4. History of old Animal Biotechnology
5. History of New Animal Biotechnology
6. Animal Biotechnology in India
7. Animal Biotechnology Research Promotion in India
8. Priorities given to Animal Biotechnology in India
9. Cloning Vectors
10. Plasmids
11. Properties of Plasmids
12. Plasmid Types
13. Properties of an Ideal Gene Cloning Vector
14. pBR 322
15. pUC8
16. Ti Plasmid
17. Disarmed Ti Plasmids
18. Bacteriophage Vectors 
19. Lambda Phage Vector
20. Insertional Vectors
21. Replacement Vectors
22. Life Cycle of Ć Phage
23. M13 Bacteriophage Vector
24. Cosmids
25. Phagemids
26. Plant Viral Vector - CaMV
27. Animal Viral Vector - SV40
28. Yeast Artificial Chromosomes (YAC)
29. Transposons as Vectors
30. Restriction Enzymes
31. Enzymes Used in Genetic Engineering
32. Restriction Modification Systems
33. Gene Cloning
34. Preparation of Desired DNA
35. Isolation of Plasmid Vector
36. Insertion of Desired Gene into Vector DNA
37. Introduction of Recombinant DNA into Host Cells
38. Introduction of rDNA into Prokaryotic Cells
39. Transfection-Introduction of rDNA into Eukaryotic Animal Cells
40. Transduction - Introduction of rDNA into Eukaryotic Animal Cells
41. Microinjection - Introduction of rDNA into Eukaryotic Animal Cells
42. Biolistics - Introduction of rDNA into Eukaryotic Animal Cells
43. Electroporation -Introduction of rDNA into Eukaryotic Animal Cells
44. Lipofection - Introduction of rDNA into Eukaryotic Animal Cells
45. Screening and Selection of Recombinants
46. Insertional Inactivation
47. Blue-white Selection
48. Direct Selection
49. Nucleic Acid Hybridization
50. Colony Hybridization
51. Blotting Techniques
52. Southern Blotting
53. Northern Blotting
54. Western Blotting 
55. Genomic Library 
56. DNA Probes
57. Polymerase Chain Reaction (PCR)
58. Animal Cell and Tissue Culture
59. History of Animal Tissue Culture
60. Requirements for Animal Cell Culture Laboratory
61. Substrates for Animal Cell Culture
62. Liquid Media for Animal Cell Culture
63. Gases for Animal Tissue Culture
64. Maintenance of Aseptic Condition
65. Cell Culture Technique
66. Isolation of Explant
67. Culture of Explants
68. Disaggregation of Explants
69. Cryopreservation - Storage of Animal Cells
70. Large - scale Culture
71. Monolayer Culture
72. Suspension - Culture for Animal Cells
73. Bioreactors
74. Continuous Stirred Tank Bioreactors (CSTB)
75. Air-Lift Bioreactors
76. Organ Culture
77. Artificial Skin
78. Artificial Cartilage
79. StemCells
80. Stem Cell Culture
81. Hybridoma Technology
82. Monoclonal Antibodies (MAbs)
83. Transgenic Animal Technology
84. Transgenic Dolly
85. Applications of Transgenic Animals
86. Gene Therapy
87. Bone Marrow Transplantation
88. Liver Transplantation
89. Enzyme Technology
90. The Production of Beta Galactosidase Enzyme
91. Enzyme Immobilization
92. Bioethics
93. Intellectual Property Rights (IPR)
94. Patenting of Biotechnological Products
95. Biosafety Guidelines in India
96. Sewage Treatment
97. Bioremediation
98. Role of Genetically Engineered Organisms in Bioremediation
99. Superbug 
100. Phytoremediation
101. Biofuels
102. Ethanol as Biofuel
103. Biogas as Biofuel
104. Aquaculture Biotechnology
105. DOT - ELISA (Enzyme Linked Immuno Sorbent Assay)
106. Human Genome Project
107. DNA Finger Printing Techniques
108. Microarray
109. Biochips
110. Bioweapons
Index
University Questions