Description

Contents
1. Introduction to Genetic Engineering
2. Concepts of Genetic Engineering
3. Methodology of Genetic Engineering
4. Construction of Recombinant DNA
5. Gene Cloning
6. Tools used in Genetic Engineering
7. Linking of Desired Gene with Vector DNA
8. Gene Cloning Vectors
9. In vitro Construction of pBR322
10. In vitro construction of Cosmid
11. Preparation of Desired DNA
12. Introduction of rDNAs into Host Cells
13. Selection of Recombinants
14. Expression of Cloned Genes
15. Polymerase Chain Reaction (PCR)
16. Blotting Techniques
17. DNA Sequencing
18. Molecular Markers and their Applications
19. Genomic Library
20. cDNA Libraries
21. Chromosome Walking
22. Chromosome Jumping
23. Genetically Engineered Microorganisms
24. Transgenic Plants
25. Transgenic Animals
26. Biohazards of Recombinant DNA Technology
27. Applications of Genetic Engineering
28. Glossary