Description

Contents1. Introduction to Genetic Engineering 2. Concepts of Genetic Engineering 3. Methodology of Genetic Engineering 4. Construction of Recombinant DNA 5. Gene Cloning 6. Tools used in Genetic Engineering 7. Linking of Desired Gene with Vector DNA 8. Gene Cloning Vectors 9. In vitro Construction of pBR322 10. In vitro construction of Cosmid 11. Preparation of Desired DNA 12. Introduction of rDNAs into Host Cells 13. Selection of Recombinants 14. Expression of Cloned Genes 15. Polymerase Chain Reaction (PCR) 16. Blotting Techniques 17. DNA Sequencing 18. Molecular Markers and their Applications 19. Genomic Library 20. cDNA Libraries 21. Chromosome Walking 22. Chromosome Jumping 23. Genetically Engineered Microorganisms 24. Transgenic Plants 25. Transgenic Animals 26. Biohazards of Recombinant DNA Technology 27. Applications of Genetic Engineering 28. Glossary