Description

Contents:1. Introduction to Biotechnology 2. Concepts of Biotechnology 3. Genetic Engineering 4. Steps in Genetic Engineering 5. Tools Used in Biotechnology 6. Enzymes Used in Genetic Engineering 7. Restriction Enzymes 8. Modification Systems 9. DNA Ligases 10. Preparation of Desired DNA 11. Linking of Desired Gene with Vector DNA 12. Gene Cloning Vectors 13. In Vitro Construction of pBR322 14. Construction of Recombinant DNA 15. Gene Cloning 16. Introduction of rDNA into Host Cells 17. Agrobacterium Mediated Gene Transfer in Plants 18. Identification and Selection of rDNA 19. Expression of Cloned Genes in Host Cells 20. Genetic Manipulation of Nif-Genes 21. Transgenic Plants 22. Transgenic Animals 23. Transgenic Microbes 24. Assisted Reproductive Technology 25. Polymerase Chain Reaction (PCR) 26. DNA Probes 27. Nucleic Acid Hybridization 28. Blotting Techniques 29. DNA Sequencing 30. DNA Library 31. DNA Fingerprinting 32. Microarray 33. Biochips 34. Biosensor 35. Glucometer 36. Human Genome Project 37. Micropropagation 38. Production of Secondary Metabolites 39. Bioreactors 40. Amino Acid Production 41. Industrial Production of Therapeutic Agents 42. Immunotherapeutic Drugs 43. Biotechnology in Health Care 44. Gene Therapy 45. Antisense RNAi Technology 46. DNA Vaccines 47. Recombinant Vaccines 48. Stem cells 49. Single Cell Proteins (SCP) 50. Mushroom Cultivation 51. Biofuels 52. Biofertilizers 53. Biopesticides 54. Glossary 55. Index