Description

1. Introduction to Plant Tissue Culture 2. Scope of Plant Tissue Culture 3. Importance of Plant Tissue Culture 4. History of Plant Tissue Culture Technology 5. Tissue Culture Laboratory Organization 6. Tissue Culture Laboratory Requirements 7. Sterilization Techniques 8. Nutrient Media 9. Composition of Nutrient Medium 10. Preparation of Stock Solutions 11. Preparation of Culture Medium 12. Types and Methods of Plant Tissue Culture 13. Totipotency of Cells 14. Callus Culture 15. Apical Meristem Culture 16. Protoplast 17. Protoplast Culture 18. Isolation of Protoplasts 19. Protoplast Fusion 20. Selection of Hybrid Protoplasts 21. Regeneration of Hybrid Plants 22. Importance of Protoplast Fusion 23. Cybrid 24. Artificial Seeds 25. Production of Artificial Seeds 26. Introduction to Genetic Engineering 27. Techniques of Genetic Engineering 28. Construction of Recombinant DNA 29. Isolation of Specific Genes 30. Enzymes Used in Gene Cloning 31. Restriction Enzymes 32. Restriction Modification Systems 33. Gene Cloning Vectors 34. Plasmids 35. Properties of Plasmids 36. Plasmid Types 37. Properties of an Ideal Gene Cloning Vector 38. pBR 322 39. pUC8 40. Ti Plasmid 41. Disarmed Ti Plasmids 42. Bacteriophage Vectors 43. Lambda Phage Vector 44. M13 Bacteriophage Vector 45. Cosmids 46. Phagemids 47. Plant Viral Vector - CaMV of Cosmid 48. cDNA Libraries 49. Agrobacterium Mediated Gene Transfer in Plants 50. DNA Transfer Techniques 51. Microinjection 52. Calcium Phosphate- Chemical Methods of DNA Transfer (Transfection) 53. Electroporation (Electrical Method of DNA Transfer) 54. Bacterial Conjugation 55. Bacterial Transformation-Natural Method of DNA Transfer 56. Genetically Modified Plants (GM Plants) 57. Bt Brinjal 58. Golden Rice 59. Bt Cotton 60. Bioethics 61. Identification or Selection of Recombinants 62. Insertional Inactivation 63. Immunological Method 64. Colony Hybridization 65. Selection of Recombinants Using Reporter Genes 66. Blotting Techniques 67. Southern Blotting 68. Northern Blotting 69. Western Blotting 70. Mutagenesis 71. Site Directed Mutagenesis 72. Random Mutagenesis