Fundamentals of Biotechnology
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ISBN 9789384826536
Pages : 576
Contents
1. Introduction to Biotechnology, 2. Concepts of Biotechnology, 3. Genetic Engineering, 4. Steps in Genetic Engineering, 5. Tools Used in Biotechnology, 6. Enzymes Used in Genetic Engineering, 7. Restriction Enzymes, 8. Modification Systems, 9. DNA Ligases, 10. Preparation of Desired DNA, 11. Linking of Desired Gene with Vector DNA, 12. Gene Cloning Vectors, 13. In Vitro Construction of pBR322, 14. Construction of Recombinant DNA, 15. Gene Cloning, 16. Introduction of rDNA into Host Cells, 17. Agrobacterium Mediated Gene Transfer in Plants, 18. Identification and Selection of rDNA, 19. Expression of Cloned Genes in Host Cells, 20. Genetic Manipulation of Nif-Genes, 21. Transgenic Plants, 22. Transgenic Animals, 23. Transgenic Microbes, 24. Assisted Reproductive Technology, 25. Polymerase Chain Reaction (PCR), 26. DNA Probes, 27. Nucleic Acid Hybridization, 28. Blotting Techniques, 29. DNA Sequencing, 30. DNA Library, 31. DNA Fingerprinting, 32. Microarray, 33. Biochips, 34. Biosensor, 35. Glucometer, 36. Human Genome Project, 37. Micropropagation, 38. Production of Secondary Metabolites, 39. Bioreactors, 40. Amino Acid Production, 41. Industrial Production of Therapeutic Agents, 42. Immunotherapeutic Drugs, 43. Biotechnology in Health Care, 44. Gene Therapy, 45. Antisense RNAi Technology, 46. DNA Vaccines, 47. Recombinant Vaccines, 48. Stem cells, 49. Single Cell Proteins (SCP), 50. Mushroom Cultivation, 51. Biofuels, 52. Biofertilizers, 53. Biopesticides, 54. Glossary, 55. Index

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